Toxic Effects of Copper Sulfate and Trichlorfon on the Intestines of Zebrafish (Danio rerio)

Copper sulfate and trichlorfon are commonly used in aquaculture. They often alone or combination to kill pathogens parasites. However, there few studies evaluating the toxic effects of copper on fish. The intestine is an important digestive immune tissue for fish, which essential growth development. In this study, zebrafish were as research model detect (0.5 mg/L) at common production concentrations fish intestine. results showed that significantly induced activities intestinal catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPX), alkaline phosphatase (AKP), but had no significant histology after short-term exposure 7 days. Following 21 days long-term exposure, could induce mucosal damage, inhibit activity antioxidant enzymes, expression tight junction protein genes 1 (ZO1), claudin (Cldn1), occludin (Oclna) These suggested probably oxidative stress injury by inhibiting damage genes. Moreover, combined was more than single suggest toxicity a synergistic effect. present should be with caution