Towards high-quality biodiesel production from microalgae using original and anaerobically-digested livestock wastewater

In this study, we conducted proof-of-concept research towards the simultaneous treatment of livestock wastewater and generation high-quality biodiesel, through microalgae technology. Both original (OPE) anaerobically-digested (DPE) piggery effluents were investigated for culture microalgae, Desmodesmus sp. EJ8-10. After 14 days’ cultivation, dry biomass from cultivated in OPE increased an initial value 0.01 g/L to 0.33–0.39 g/L, while those growing DPE only achieved a final dried mass 0.15–0.35 under similar ammonium nitrogen (NH 4 + -N) concentrations. The significantly higher microalgal production medium may have been supported by abundance both macronutrient, such as phosphorus (P), micronutrients, trace elements, present , which not quantities DPE. However, lipid content was observed (19.4–28%) cells cultures than (18.7–22.3%) cultures. Moreover, fatty acid compositions cultured contained high levels monounsaturated acids (MUFAs) total C16–C18 acids, would afford superior potential biodiesel production. N/P ratio (15.4:1) much closer that indicated previous studies be most suitable (16:1) growth, when compared with determined medium. This facilitate protein synthesis algal induce lower accumulation lipids. Based on these findings, proposed new flowsheet sustainable waste management. • Microalgal growth hindered digested effluent culture. Microalgae removed >90% N P (OPE). could increase productivity microalgae. had better quality biofuel OPE. A recovery.