Torsionally constrained DNA for single-molecule assays: an efficient, ligation-free method
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چکیده
منابع مشابه
Torsionally constrained DNA for single-molecule assays: an efficient, ligation-free method
Controlled twisting of individual, double-stranded DNA molecules provides a unique method to investigate the enzymes that alter DNA topology. Such twisting requires a single DNA molecule to be torsionally constrained. This constraint is achieved by anchoring the opposite ends of the DNA to two separate surfaces via multiple bonds. The traditional protocol for making such DNA involves a three-wa...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2013
ISSN: 1362-4962,0305-1048
DOI: 10.1093/nar/gkt699