Topography of a vacuolar-type H+-translocating ATPase: chromaffin-granule membrane ATPase I
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منابع مشابه
Topography of a vacuolar-type H+-translocating ATPase: chromaffin-granule membrane ATPase I.
Proteins exposed on the cytoplasmic face of isolated chromaffin granules were labelled by lactoperoxidase-catalysed radioiodination and by non-enzymic biotinylation. Granule membranes were then prepared, and the H+-translocating ATPase isolated by fractionation with Triton X-114. The labelling of individual ATPase subunits was assessed by polyacrylamide-gel electrophoresis, followed by autoradi...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1989
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2630081