Topographic and active-site studies on bovine rhodopsin
نویسندگان
چکیده
منابع مشابه
A convenient synthesis of labelled rhodopsin and studies on its active site.
Digitonin solutions of labelled rhodopsin, containing (3)H in the retinyl moiety, were prepared by two related methods. Labelled rhodopsin was also prepared for the first time in cetyltrimethylammonium bromide and purified by column chromatography. It was shown that only certain rhodopsin preparations on denaturation in the dark and the reduction with sodium borohydride gave up to 60% of the ra...
متن کاملThe active-site environment of rhodopsin.
The 11-cis-retinal binding site of rhodopsin is of great interest because it is buried in the membrane but yet must provide an environment for charged amino acids. In addition, the active-site lysine residue must be able to engage in rapid Schiff base formation with 11-cis-retinal at neutral and lower pH values. This requires that this lysine be unprotonated. We have begun to study the environm...
متن کاملActive site-directed inactivation of constitutively active mutants of rhodopsin.
Recently, mutations of the active site Lys296 residue in rhodopsin (Lys296-->Glu and Lys296-->Met) have been found as the cause of disease in some patients with autosomal dominant retinitis pigmentosa. In vitro, these mutations result in constitutive activation of the protein. In an effort to develop a potential therapeutic agent for treatment of the disease, we have examined various amine deri...
متن کاملStudies on the Active Site of Clostripain
Clostripain is rapidly and selectively inactivated by the chloromethyl ketone derived from cr-N-tosyl-L-lysine (TLCK) with an apparent second order rate constant of 8.7 X lo4 M-I set-i at pH 6.5. Alkylation by TLCK occurs only with catalytically active enzyme, the rate of which is considerably retarded by the competitive inhibitors, oc-Ntosyl-L-homoarginine methyl ester and benzamidine. In cont...
متن کاملStudies on the Active Site of Chymotrypsin.
as it rises in b to enter c above the midline, etc. It can be easily seen that a similar oscillation about the midline takes place in the rotating fluid annulus of Figure 4a and in the more general situation of Figure 4b. These oscillations can be made quite small by adjusting the density of the buffer to match the particle density and by speeding up the rate of buffer flow to shorten the perio...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1981
ISSN: 0014-5793
DOI: 10.1016/0014-5793(81)81174-x