TMEM189 as a target gene of MiR-499a-5p regulates breast cancer progression through the ferroptosis pathway

miR-499a-5p has been reported to regulate the progression of various tumours. However, role in breast cancer is unclear. The purpose this study was investigate and mechanism cancer. growth effect on cells investigated by CCK-8 assay, wound healing assay Transwell invasion assay. luciferase activity used verify downstream targets miR-499a-5p. levels GSH, MDA ROS were detected kits. Quantitative real-time PCR (qRT-PCR) Western blot determine expression TMEM189, COX-2, GPX4 other related genes cells. down-regulated MDA-MB-231 shown reduced viability, migration Further studies revealed that TMEM189 a target inhibited cell downregulating TMEM189. Furthermore, down-regulation promotes ferroptosis low development through pathway. We have demonstrated for first time inhibits targeting TMEM189-mediated may be effective molecular treatment