Tissue Engineering Auricular Cartilage Using Late Passage Human Auricular Chondrocytes
نویسندگان
چکیده
منابع مشابه
Cartilage tissue engineering using human auricular chondrocytes embedded in different hydrogel materials.
To seek a suitable scaffold for cartilage tissue engineering, we compared various hydrogel materials originating from animals, plants, or synthetic peptides. Human auricular chondrocytes were embedded in atelopeptide collagen, alginate, or PuraMatrix, all of which are or will soon be clinically available. The chondrocytes in the atelopeptide collagen proliferated well, while the others showed n...
متن کاملBioengineering Human Scaffold-free Cartilage Constructs Using Paediatric Auricular Tissue
Currently, patients with external ear deformities rely on hyaline cartilage grafts or silicone prosthesis to provide them with adequate external ear reconstruction. Scaffold-free auricular tissue engineering provides a means of creating patient specific cartilage constructs solely using their own cells, ensuring biocompatibility, long term stability, and reduced morbidity. Here, we have develop...
متن کاملElectrospinning Auricular Shaped Scaffolds for Tissue Engineering.
Poly(ɛ)caprolactone scaffolds have been electrospun directly into an auricular shaped conductive mould. Bovine chondrocytes were harvested from articular cartilage and seeded onto 16 of the produced scaffolds, which received either an ethanol (group A) or a plasma treatment (group B) for sterilisation before seeding. The seeded scaffolds were cultured for 3 weeks in vitro and analysed with rega...
متن کاملCartilage tissue engineering using cryogenic chondrocytes.
OBJECTIVE To generate in vitro hyaline cartilage from cryogenically preserved human septal chondrocytes in a simulated microgravity environment on a 3-dimensional biodegradable scaffolding material. METHODS In this experiment, cryogenically frozen chondrocytes were thawed and cultured in a monolayer in serum-based chondrocyte media. They were seeded onto 3-dimensional biopolymer scaffolds in ...
متن کاملCartilage tissue engineering using pre-aggregated human articular chondrocytes.
In this study, we first aimed at determining whether human articular chondrocytes (HAC) proliferate in aggregates in the presence of strong chondrocyte mitogens. We then investigated if the aggregated cells have an enhanced chondrogenic capacity as compared to cells cultured in monolayer. HAC from four donors were cultured in tissue culture dishes either untreated or coated with 1% agarose in t...
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ژورنال
عنوان ژورنال: Annals of Plastic Surgery
سال: 2018
ISSN: 0148-7043
DOI: 10.1097/sap.0000000000001400