TIMP-2 and IGFBP7 in human kidney biopsies in renal disease

ABSTRACT Background Tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) and insulin-like growth factor binding protein 7 (IGFBP7) are markers tubular stress urinary [TIMP-2]*[IGFBP7] is an established biomarker for risk assessment acute kidney injury. There no studies expression profiles or localization these in human renal tissue with confirmed disease. Methods We analysed 37 biopsies patients disease 10 non-diseased control TIMP-2 IGFBP7 using immunohistochemistry. Changes glomerular morphology were evaluated by a semi-quantitative glomerulosclerosis score (GSI) interstitial changes graded the injury (TSI) periodic acid–Schiff-stained paraffin sections. Interstitial fibrosis atrophy (IF/TA) according to Banff classification. Urinary was collected at time biopsy. Results had significantly greater from compared tissue, especially compartment. Here, detected proximal distal tubules while predominantly localized collecting ducts. Renal correlated staining intensity IGFBP7: GSI weakly (r = 0.36) 0.35) TSI 0.41) 0.43). histopathological damage but not expression. Conclusion Our findings underline role TIMP-2/IGFBP7 as unspecific marker that already use early detection