Time-lapse imaging system with shell-less culture chamber
نویسندگان
چکیده
منابع مشابه
Visualizing Bacterial Colony Morphologies Using Time-Lapse Imaging Chamber MOCHA
Capturing microbial growth on a macroscopic scale is of great importance to further our understanding of microbial life. However, methods for imaging microbial life on a scale of millimeters to centimeters are often limited by designs that have poor environmental control, resulting in dehydration of the agar plate within just a few days. Here, we created MOCHA (microbial chamber), a simple but ...
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INTRODUCTION By their very nature, biological systems are dynamic, and a proper understanding of the cellular and molecular processes underlying living organisms and how to manipulate them is a prerequisite to combat diseases and improve human health care. One of the major challenges of current biomedical research, therefore, is to unravel not just the spatial organization of these complex syst...
متن کاملTime-Lapse Imaging
By their very nature, biological systems are dynamic, and a proper understanding of the cellular and molecular processes underlying living organisms and how to manipulate them is a prerequisite to combat diseases and improve human health care. One of the major challenges of current biomedical research, therefore, is to unravel not just the spatial organization of these complex systems, but thei...
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Numerous tissue culture chambers have been devised with the object of providing the optical requirements necessary for microscopic observation of the culture, and some of these incorporate a means of perfusion to facilitate changing medium, or to introduce various agents during the period of observation (Carrel, 1931 ; de Haan, 1931, 1938; Lindbergh, 1939; Parker, 1950). A few have been designe...
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Osteoblastic mineralization occurs during the early stages of bone formation. During this mineralization, hydroxyapatite (HA), a major component of bone, is synthesized, generating hard tissue. Many of the mechanisms driving biomineralization remain unclear because the traditional biochemical assays used to investigate them are destructive techniques incompatible with viable cells. To determine...
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ژورنال
عنوان ژورنال: Development, Growth & Differentiation
سال: 2014
ISSN: 0012-1592
DOI: 10.1111/dgd.12132