منابع مشابه
Radioimmunoassay for human thyroxine-binding prealbumin.
A radioimmunoassay (RIA) for human thyroxine-binding prealbumin (PA) is described. It employs highly purified PA, anti-human PA serum at 1:30,000 final dilution, normal bovine serum as a carrier, and polyethyleneglycol to precipitate the immune complexes. This assay is extremely sensitive (limit of detection less than 0.2 micrograms per dL or less than 3.6 X 10(-15) moles per tube), accurate (r...
متن کاملThyroxine-binding prealbumin in thyrotoxicosis and hypothyroidism.
THYROXINE is reversibly bound in the plasma to three proteins: thyroxine-binding globulin (TBG), thyroxine-binding prealbumin (TBPA), and albumin. Changes in the binding capacities of TBG and TBPA in patients with thyroid disease have been reported previously (1, 2). Since starch-gel electrophoresis of serums of patients with thyrotoxicosis indicated a marked decrease in density of the stain in...
متن کاملThe interaction of thyroxine with human plasma prealbumin and with the prealbumin-retinol-binding protein complex.
Prealbumin was isolated from human plasma by chromatography on columns of diethylaminoethyl Sephadex and Sephadex G-200, followed by preparative polyacrylamide gel electrophoresis. The prealbumin was homogeneous in the analytical ultracentrifuge with an s=z~,~ of 3.7 S and with a molecular weight of about 50,000. Prealbumin formed a protein-protein complex with plasma retinol-binding protein in...
متن کاملThe Contribution of Thyroxine - Binding Prealbumin to the Binding of Thyroxine in Human Serum , as Assessed by Immunoadsorption KENNETH
KTBPA, 2.3 X 108; and KTBG, 1.7 X 1010. t Deceased 26 January 1968. Received for publication 12 November 1968 and in revised form 7 February 1968. INTRODUCTION The existence of L-thyroxine (T4)-binding prealbumin (TBPA) as a normal constituent of human serum has been satisfactorily demonstrated both by a variety of electrophoretic techniques and by its isolation from human serum (1-3). Neverthe...
متن کاملThe contribution of thyroxine-binding prealbumin to the binding of thyroxine in human serum, as assessed by immunoadsorption.
An immunoadsorption technique employing a rabbit antiserum specific for human serum prealbumin has been devised to remove thyroxine (T(4))-binding prealbumin (TBPA) from serum completely without affecting the T(4)-binding activity of thyroxine-binding globulin (TBG) or the concentration of the other major proteins in serum. As judged from the proportion of T(4) associated with the antigen-antib...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)61827-7