Three-dimensional spheroid culture targeting versatile tissue bioassays using a PDMS-based hanging drop array
نویسندگان
چکیده
منابع مشابه
Hanging Drop Tissue Culture
The hanging drop tissue culture is a technique utilized in embryology [4] and other fields to allow growth that would otherwise be restricted by the flat plane of culture dishes and also to minimize the surface area to volume ratio, slowing evaporation. The classic hanging drop culture is a small drop of liquid, such as plasma or some other media allowing tissue growth, suspended from an invert...
متن کاملPaper-Supported Three-Dimensional Cell Culture for Tissue-Based Bioassays
Methods. Cells and reagents We purchased MDA-MB-231 cells from ATCC and primary IMR90, HDF and primary HUVEC cells from Lonza. MDA-MB-231, IMR-90, HDF and LLC cells were cultured in Eagle’s minimal Essential medium (MEM) from Lonza supplemented with 10% fetal bovine serum (FBS) and 1% GlutaMax (both from Invitrogen). Primary HUVEC cells were cultured in endothelial growth medium (EGM-2, Lonza)....
متن کاملA Microfluidic Hanging Drop Chip for Automated Culture of Three - dimensional Cell Aggregates
The hanging drop (HD) cell culture method is widely used for the formation of in vitro three-dimensional (3D) cell aggregates, avoiding the cellular distortion typically seen in the two-dimensional cell culture. The conventional HD technique consists of placing small drops of cell suspension solution on a plastic substrate, inverting the substrate, and incubating for the desired or permitted le...
متن کاملReassembly of Anterior Pituitary Organization by Hanging Drop Three-Dimensional Cell Culture
The anterior pituitary gland comprises 5 types of hormone-producing cells and non-endocrine cells, such as folliculostellate (FS) cells. The cells form a lobular structure surrounded by extracellular matrix (ECM) but are not randomly distributed in each lobule; hormone-producing cells have affinities for specific cell types (topographic affinity), and FS cells form a homotypic meshwork. To dete...
متن کاملA PDMS-Based Microfluidic Hanging Drop Chip for Embryoid Body Formation.
The conventional hanging drop technique is the most widely used method for embryoid body (EB) formation. However, this method is labor intensive and limited by the difficulty in exchanging the medium. Here, we report a microfluidic chip-based approach for high-throughput formation of EBs. The device consists of microfluidic channels with 6 × 12 opening wells in PDMS supported by a glass substra...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2017
ISSN: 2045-2322
DOI: 10.1038/s41598-017-04718-1