Three-Dimensional Printed Abdominal Imaging Windows for In Vivo Imaging of Deep-Lying Tissues

The ability to microscopically image diseased or damaged tissue throughout a longitudinal study in living mice would provide more insight into disease progression than having just couple of time points study. In vivo development and monitoring provides vitro studies as well. this study, we developed permanent 3D-printed, surgically implantable abdominal imaging windows (AIWs) allow for deep-lying tissues organs the cavity mice. They are designed prevent organ movement while allowing animal behave normally studies. AIW also acts its own mounting bracket attaching them custom 3D printed microscope mount that attaches stage houses inside. During animal, cellular macroscopic changes over one location can be observed because markers used find same spot each session. We were able deliver cancer cells pancreas use progression. design AIWs expanded include secondary features, such delivery manipulation ports guides, make brain, subcutaneous implants, mammary tissue. all, these 3D-printed their system enhancing animals during