Three-Dimensional Cell Culture and Tissue Engineering in a T-CUP (Tissue Culture Under Perfusion)

Three-dimensional cell culture and tissue engineering in a T-CUP (tissue culture under perfusion).

The aim of this study was to develop and validate a simple and compact bioreactor system for perfusion cell seeding and culture through 3-dimensional porous scaffolds. The developed Tissue Culture Under Perfusion (T-CUP) bioreactor is based on the concept of controlled and confined alternating motion of scaffolds through a cell suspension or culture medium, as opposed to pumping of the fluid th...

Three-dimensional tissue culture based on magnetic cell levitation.

Cell culture is an essential tool in drug discovery, tissue engineering and stem cell research. Conventional tissue culture produces two-dimensional cell growth with gene expression, signalling and morphology that can be different from those found in vivo, and this compromises its clinical relevance. Here, we report a three-dimensional tissue culture based on magnetic levitation of cells in the...

Paper-Supported Three-Dimensional Cell Culture for Tissue-Based Bioassays

Methods. Cells and reagents We purchased MDA-MB-231 cells from ATCC and primary IMR90, HDF and primary HUVEC cells from Lonza. MDA-MB-231, IMR-90, HDF and LLC cells were cultured in Eagle’s minimal Essential medium (MEM) from Lonza supplemented with 10% fetal bovine serum (FBS) and 1% GlutaMax (both from Invitrogen). Primary HUVEC cells were cultured in endothelial growth medium (EGM-2, Lonza)....

Cell and Tissue Culture

An Easily Made Tissue Culture Perfusion Chamber

A perfusion chamber is described with which a tissue culture can be irrigated successively with different immersion media. Only 1-2 ml. of medium are required for each change, which can be accomplished within 1 minute. Simplicity of design permits speed and economy in manufacture. Perfusion by suction instead of by positive pressure has eliminated certain drawbacks of previous designs. Mixing e...