Three Decades of Advances in Arabinogalactan-Protein Biosynthesis

Extensin and Arabinogalactan-Protein Biosynthesis: Glycosyltransferases, Research Challenges, and Biosensors

Recent research, mostly in Arabidopsis thaliana, has led to the identification and characterization of the glycosyltransferases responsible for the biosynthesis of two of the most functionally important and abundant families of plant cell wall proteins, extensins, and arabinogalactan-proteins. Extensin glycosylation involves monogalactosylation of serine residues by O-α-serine galactosyltransfe...

Functional identification of a hydroxyproline-o-galactosyltransferase specific for arabinogalactan protein biosynthesis in Arabidopsis.

Although plants contain substantial amounts of arabinogalactan proteins (AGPs), the enzymes responsible for AGP glycosylation are largely unknown. Bioinformatics indicated that AGP galactosyltransferases (GALTs) are members of the carbohydrate-active enzyme glycosyltransferase (GT) 31 family (CAZy GT31) involved in N- and O-glycosylation. Six Arabidopsis GT31 members were expressed in Pichia pa...

Three Decades of Multiprocessors

During the last 25 years, the author has never reallv considered any alternative to the multiprocessor (mP) for general-purpose, cost-effective, nonminimal come -rs. This involvement with mPs at Digital Equipment Corporation (DEC), Carnegie Mellon University (CMU), Encore, Stardent, and Kendall Square Research (KSR) included 16 computers. Fourteen were built, including 7 for research or advance...

Advances and challenges in recombinant protein expression in Escherichia coli

Biosynthesis of the arabinogalactan-peptidoglycan complex of Mycobacterium tuberculosis.

The compositional complexity of the mycobacterial cell envelope differentiates Mycobacterium species from most other prokaryotes. Historically, research in this area has focused on the elucidation of the structure of the mycobacterial cell envelope with the result that the structures of the mycolic acid-arabinogalactan-peptidoglycan complex from M. tuberculosis are fairly well understood. Howev...