Thiopalmitoylation of Myelin Proteolipid Protein Epitopes Enhances Immunogenicity and Encephalitogenicity
نویسندگان
چکیده
منابع مشابه
Thiopalmitoylation of myelin proteolipid protein epitopes enhances immunogenicity and encephalitogenicity.
Proteolipid protein (PLP) is the most abundant protein of CNS myelin, and is posttranslationally acylated by covalent attachment of long chain fatty acids to cysteine residues via a thioester linkage. Two of the acylation sites are within epitopes of PLP that are encephalitogenic in SJL/J mice (PLP(104-117) and PLP(139-151)) and against which increased immune responses have been detected in som...
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multiple sclerosis (ms) is an autoimmune disease in which auto-reactive t cells react with self-antigens expressed in the central nervous system (cns). the main cause of ms is unknown. nonetheless, the most probable theory is based on molecular mimicry, which suggests that some infections can activate t cells against brain auto-antigens like myelin proteolipid protein (plp) and initiate the dis...
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Myelin basic proteins (MBPs) from 6-day-old, 10-day-old, 20-day-old and adult normal mouse brain were compared with those from 20-day-old jimpy (dysmyelinating mutant) mouse brain to determine the effect of reduced levels of proteolipid protein (PLP) on MBPs. Alkaline-urea-gel electrophoresis showed that 6-day-old and 10-day-old normal and jimpy MBPs lacked charge microheterogeneity, since C8 (...
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Two proteolipid proteins, PLP and DM20, are the major membrane components of central nervous system (CNS) myelin. Mutations of the X-linked PLP/DM20 gene cause dysmyelination in mouse and man and result in significant mortality. Here we show that mutant mice that lack expression of a targeted PLP gene fail to exhibit the known dysmyelinated phenotype. Unable to encode PLP/DM20 or PLP-related po...
متن کاملCharacterization of proteolipid protein fatty acylesterase from rat brain myelin.
A protein fatty acylesterase activity that catalyzes the removal of fatty acid from exogenous proteolipid protein (PLP) has been demonstrated in isolated rat brain myelin. Optimum enzyme activity for the deacylation of PLP was obtained in 0.5% Triton X-100, 1 mM dithiothreitol at pH 7.0 and at 37 degrees C. Other detergents (octyl beta-D-glucoside, Nonidet P-40, and Tween 20) have little or no ...
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ژورنال
عنوان ژورنال: The Journal of Immunology
سال: 2001
ISSN: 0022-1767,1550-6606
DOI: 10.4049/jimmunol.166.11.6907