Thin-layer chromatography of tissue lipids without extraction

Thin-layer chromatography of tissue lipids without extraction.

Frozen tissue sections were applied directly to silica gel plates, and the lipids were separated by developing the plates in different solvent systems. Quantitation of the lipid classes was achieved by direct transmission densitometry after the plates were treated with a chromic acid-sulfuric acid spray.

Thin-layer Chromatography of Lipids

There has been a tendency to discount thin-layer chromatography (TLC) in recent years because of the availability of high-performance liquid chromatography (HPLC) methods that appeared to offer greater versatility and ease of quantification, amongst other reasons. However, TLC has made a comeback, largely because of the high reproducibility and lower costs of commercial pre-coated plates. It is...

Two dimensional thin-layer chromatography of polar lipids.

A two-dimensional thin-layer chromatographic system that separates all of the known polar lipids of milk and mammary tissue is described. The compositions of phospholipids of milk and of the mammary tissue from which the milk was derived are presented.

Separation of plasma lipids by thin-layer chromatography.

A rapid comprehensive thin-layer chromatographic separation of the major plasma lipid classes on commercial silicic acid glass fiber paper is described. For screening purposes, oxidized fractions are rapidly visualized for qualitative interpretation. Standard colorimetric procedures for each lipid class are per­ formed upon the migration areas cut from the strips. This provides quantitative mea...

A Simple Method for the Separation of Minute Amounts of Tissue Lipids by Thin-layer Chromatography and Gas-liquid Chromatography.