Thieno[2,3-b]Pyridine Derivative Targets Epithelial, Mesenchymal and Hybrid CD15s+ Breast Cancer Cells

The adhesion of cancer cells to vascular endothelium is a critical process in hematogenous metastasis and might be similar the recruitment leukocytes at site inflammation. It mediated by E-selectin its ligands, which most stereospecific glycoconjugate sialyl Lewis x (CD15s), may expressed as an oligosaccharide branch CD44 glycoprotein, well self-contained glycosphingolipid. also known that increased sialylation glycoconjugates feature malignant cells. aim study was analyse effect novel thieno[2,3-b]pyridine, compound 1, MDA-MB-231 triple-negative breast (TNBCs) upon CD15s expression different cell subpopulations using flow cytometry. compared between mesenchymal-like stem (CSC, CD44+CD24−), epithelial without (CD44−CD24+ CD44−CD24−), CD44+CD24+ exhibit mesenchymal features. In addition, CD15s+CSC CD15s−CSC determined. Compound 1 significantly decreased percentage CD15s+CSC, CD15s+CD44+CD24+, CD15s+CD44− subpopulations, CD44− cells, therefore shows potential treatment for TNBC.