The Thiobarbituric Acid Assay of Sialic Acids
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چکیده
منابع مشابه
The thiobarbituric acid assay of sialic acids.
Members of the sialic acid family of compounds occur in a variety of tissues and body fluids (la, 2) and in bacteria (3), incorporated in conjugated protein. The naturally occurring sinlic acids are substituted neuraminic acid derivatives (Nacetyl, N-glycolyl, N, 0-diacetylneuraminic acid). These are collectively termed “sialic acids.” The unsubstituted 9 carbon chain is called neuraminic acid ...
متن کاملDirect assay of bound sialic acids
Bound sialic acids on rat spermatozoa were assayed by oxidation with 1 mMNaIO4 at 0\s=deg\C,liberating C-9 as formaldehyde which was further quantitated using 3\x=req-\ methyl-2-benzothiazolinone. The mean \m=+-\s.d. (n = 20) content of bound sialic acids of spermatozoa from the caput and cauda epididymidis was 50\m=.\9 \m=+-\8\m=.\0and 25\m=.\2 \m=+-\3\m=.\8 nmol/108 spermatozoa respectively. ...
متن کاملStable thiobarbituric acid chromophore with dimethyl sulphoxide. Application to sialic acid assay in analytical de-O-acetylation.
With dimethyl sulphoxide instead of butanol in the thiobarbituric acid assay for sialic acid, a non-fading chromophore with lambdamax. = 549 nm was produced in a homogeneous solution, allowing dilution of the test mixture in case of high colour yield. This test adapted well to studies on alkaline de-O-acetylation. Bovine and rat submaxillary mucins, and rabbit Tamm-Horsfall urinary sialoprotein...
متن کاملElectrochemical Study of Iodide in the Presence of 2-Thiobarbituric Acid-Catalytic Determination of 2-Thiobarbituric Acid
Electrochemical oxidation of iodide has been studied in the presence of 2-thioborbituric acid using cyclic voltammetry and controlled-potential coulometry. The results indicate that, the resulting iodine takes part in halogenations reaction with 2-thiobarbituric acid. In addition, the present data are indicative of the suitability of iodide as a quasi mediator for determination of 2-thiobar...
متن کاملThe biosynthesis of sialic acids.
The sialic acids (1) have in common a 9 carbon carboxylic acid, neuraminic acid (Fig. l), which may be regarded as a condensation product of a 3 carbon and a 6 carbon unit. Neuraminic acid itself is not found naturally, but several Nand O-substituted derivatives are widely distributed in nature (1). Chemical (2-4) and enzymic (5-7) degradations of N-acetylneuraminic acid have shown that this su...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1959
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)69851-5