The stromal vascular fraction mitigates radiation-induced gastrointestinal syndrome in mice

Abstract Background The intestine is particularly sensitive to moderate-high radiation dose and the development of gastrointestinal syndrome (GIS) leads rapid loss intestinal mucosal integrity, resulting in bacterial infiltration, sepsis that comprise patient survival. There an urgent need for effective therapeutic countermeasures. stromal vascular fraction (SVF) derived from adipose tissue easily accessible source cells with angiogenic, anti-inflammatory regenerative properties. We studied impact SVF its action on stem cell compartment. Methods Mice exposed abdominal (18 Gy) received a single intravenous injection (2.5 × 10 6 cells), obtained by enzymatic digestion inguinal fat tissue, day irradiation. Mortality was evaluated as well regeneration histological analyses absorption function. Results treatment limited weight mice inhibited permeability mortality after Histological showed stimulated epithelium promoting numerous enlarged hyperproliferative zones. restored CD24 + /lysozyme − Paneth populations ISC compartment presence Ki67 cells. has effect repressing pro-inflammatory cytokines, increasing M2 macrophages ileum monocyte subtypes CD11b Ly6c low CX3CR1 high spleen. Conclusions Through pleiotropic effects contribute limiting radiation-induced lethality, opens up attractive prospects emergency GIS.