THE STREPTOCOCCUS FAECALIS OXIDASES FOR REDUCED DIPHOSPHOPYRIDINE NUCLEOTIDE
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چکیده
منابع مشابه
The reduced diphosphopyridine nucleotide oxidase of Streptococcus faecalis: purification and properties.
Extracts of anaerobically grown cells of Streptococcus fuecalis, strain lOC1, contain a reduced diphosphopyridine nucleotide oxidase which catalyzes the reduction of oxygen to hydrogen peroxide (1, 2). Although the enzyme has not been purified, experiments (3) carried out with extracts of the organism grown in riboflavin-deficient media, as well as experiments in which acid-salt resolution of t...
متن کاملEnzymatic conversion of a reduced diphosphopyridine nucleotide derivative to reduced diphosphopyridine nucleotide.
DPNH-X was prepared as described previously (1) ; the barium salt was converted to the sodium salt before use, and solutions were stored at 20”. Concentrations of DPNH-X were calculated with an extinction coefficient of 13.5 X lo6 sq. cm. mole-’ at 290 rnM (1). Sodium salts of ADP, ATP, ITP, UTP, and crystalline disodium ATP were obtained from the Pabst Laboratories, Milwaukee, Wisconsin. Myoki...
متن کاملAutomated assay of activities of enzymes involving the diphosphopyridine nucleotide-reduced diphosphopyridine nucleotide reaction.
A continuous flow instrumental system of analysis, first described by Skeggs (l), was previously applied to the determination of phosphohexose isomerase activity and resulted in a method that was approximately eightfold as rapid as the manual procedure (2). The present paper is concerned with the application of this system of analysis to the determination of enzyme activities that utilize the d...
متن کاملThe mechanism of triosephosphate dehydrogenase inactivation by reduced diphosphopyridine nucleotide.
We have shown in a previous communication (1) that the enzyme triosephosphate dehydrogenase is rapidly inactivated in the presence of reduced diphosphopyridine nucleotide at physiological concentrations, temperature, and pH. Good stoichiometry was shown for the amount of enzyme inactivation, DPNH-X1 formation, and disappearance of sulfhydryl groups. It was then of particular interest to determi...
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From the particulate reduced nicotinamide adenine dinucleotide (DPNH) oxidase of beef heart, Mackler (3) has obtained a soluble DPNH dehydrogenase which was estimated to be approximately 90 to 95% homogenous when examined electrophoretically.l Similarly, when the enzyme was chromatographed on diethylaminoethyl cellulose with phosphate buffer, pH 7.5, in a gradient elution method (0.001 M to 0.0...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1957
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)64952-x