منابع مشابه
The soft agar colony formation assay.
Anchorage-independent growth is the ability of transformed cells to grow independently of a solid surface, and is a hallmark of carcinogenesis. The soft agar colony formation assay is a well-established method for characterizing this capability in vitro and is considered to be one of the most stringent tests for malignant transformation in cells. This assay also allows for semi-quantitative eva...
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The fibroblast mitogen platelet-derived growth factor -BB (PDGF-BB) induces a transient expression of the orphan nuclear receptor NR4A1 (also named Nur77, TR3 or NGFIB). The aim of the present study was to investigate the pathways through which NR4A1 is induced by PDGF-BB and its functional role. We demonstrate that in PDGF-BB stimulated NIH3T3 cells, the MEK1/2 inhibitor CI-1040 strongly repre...
متن کاملCFAssay: statistical analysis of the colony formation assay
BACKGROUND Colony formation assay is the gold standard to determine cell reproductive death after treatment with ionizing radiation, applied for different cell lines or in combination with other treatment modalities. Associated linear-quadratic cell survival curves can be calculated with different methods. For easy code exchange and methodological standardisation among collaborating laboratorie...
متن کاملRapid assay for evaluating the chemosensitivity of human tumors in soft agar culture.
Assays that measure [3H]thymidine incorporation by cells plated in soft agar were investigated to identify a rapid method for assessing Chemosensitivity of tumor cells. Six established cell lines (five melanomas and one colon carcinoma) and cells prepared from 23 primary or metastatic tumors (ten melano mas, five colon adenocarcinomas, three lung carcinomas, two ovarian adenocarcinomas, one bre...
متن کاملPreparation of permanent slides of intact soft-agar colony cultures of hematopoietic and tumor stem cells.
A simple technique is described for fixing colony-containing layers of soft agar and drying them onto microscopic slides. The method is extrapolated from techniques used in immunology for permanent preservation of immunodiffusion or immunoelectrophoresis plates. Slides prepared in this fashion are eminently suitable for subsequent analysis with a variety of techniques including conventional Pap...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2014
ISSN: 1940-087X
DOI: 10.3791/51998