The Separation of Polythionates with Anion-Exchange Resins

Regeneration Studies of Anion-Exchange Chromatography Resins

LEVEL: INTERMEDIATE C hromatography columns can become contaminated by a variety of protein and nonprotein species during a purification campaign. Consequences of column contamination include an increase in backpressure, loss of signal resolution, altered product yield, and medium discoloration. Common chromatographic contaminants include • residual proteins • nucleic acids • lipids • endotoxin...

Displacement chromatography on synthetic ion-exchange resins. 2. The separation of organic acids and acidic amino-acids by the use of anion-exchange resins.

The application of the principle of displacement chromatography to the separation of bases and amino-acids on columns packed with the cationexchange resin Zeo-Karb 215 has been described in Part 1 of this communication (Partridge & Westall, 1949). It is expected that this method will prove to be of value in the isolation of bases and amino-acids from biological extracts of various kinds, and th...

Recycling of anion-exchange resins for plasmid DNA purification.

Plasmid DNA of high purity is critical for molecular biology studies such as subcloning, DNA sequencing, vector construction and expression and functional analysis. The classical method of plasmid DNA purification using cesium chloride/ethidium bromide buoyant-density centrifugation is generally time-consuming and expensive and has gradually been replaced by the use of silica-based, anion-excha...

Speciation Modeling Of LREEs-NTA Complexes On Anion- Exchange Resins

Conventionally, the sorption methods of rare earth elements were done with cationic ion exchangers. The present work was mainly oriented to find an alternative method for sorption of light rare earth elements (LREEs) based on anionic ion exchangers. Two different anionic exchangers have been tested including Dowex-1 and Sybron SR-7. The selection of these two exchangers was mainly done to inves...

Trace contamination following reuse of anion-exchange DNA purification resins.