The separation of heavy meromyosin isoenzymes by differential actin binding
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چکیده
منابع مشابه
The Localization of Actin-like Fibers in Cultured Neuroblastoma Cells as Revealed by Heavy Meromyosin Binding
Actin-like protein has been isolated from cultured since normal cell morphology is disrupted during sympathetic ganglia of chick (6) and mammalian glycerination, the exact localization of actinbrain (18), and accounts for up to 207 of the total HMM complexes in axons and cell bodies has not cell protein (6) . The exact ultrastructural localizabeen ascertained . tion of such a large amount of ac...
متن کاملFunction of Heavy Meromyosin in the Acceleration of Actin Polymerization.
The function of myosin A in the acceleration of actin polymerization was first observed by Laki and Clark (1) who noted the catalytic effect of myosin A. Martonosi and Gouvea (2) used a light scattering method to demonstrate that an enzymically active fragment of myosin A, heavy meromyosin, prepared by tryptic digestion, also accelerated the actin polymerization. Yagi and Sakakibara (3) have sh...
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Relaxation of both smooth and skeletal muscles appears to be caused primarily by inhibition of the step associated with Pi release in the actomyosin ATPase cycle, rather than by a block in the binding of the myosin X ATP and myosin X ADP X Pi complexes to actin. In skeletal muscle, troponin-tropomyosin not only causes marked inhibition of Pi release, but it also markedly inhibits the binding of...
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Consden, R. & Gordon, A. H. (1948). Nature, Lond., 162, 180. Dixon, J. S. & Lipkin, D. (1954). Analyt. Chem. 26, 1092. Elliott, S. D. (1962). Nature, Lond., 193, 1105. Foster, A. B., Horton, D. & Stacey, M. (1957). J. chem. Soc. p. 81. Gelotte, B. (1960). J. Chromat. 3, 330. Hanahan, D. J. & Olley, J. N. (1958). J. biol. Chem. 231, 813. Hanes, C. S. & Isherwood, F. A. (1949). Nature, Lond., 164...
متن کاملActin in dividing cells: contractile ring filaments bind heavy meromyosin.
Many microfilaments and microtubules are well preserved after glycerol-extraction of HeLa cells at room temperature (22 degrees ). Incubation in heavy meromyosin from rabbit skeletal muscle results in conspicuous and characteristic "decoration" of microfilaments of the contractile ring. Decoration is completely prevented by 10 mM ATP or 2 mM pyrophosphate, and fails to occur if heavy meromyosin...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1977
ISSN: 0014-5793
DOI: 10.1016/0014-5793(77)80659-5