The RSC complex remodels nucleosomes in transcribed coding sequences and promotes transcription in <i>Saccharomyces cerevisiae</i>

Abstract RSC (Remodels the Structure of Chromatin) is a conserved ATP-dependent chromatin remodeling complex that regulates many biological processes, including transcription by RNA polymerase II (Pol II). We report contributes in generating accessible nucleosomes transcribed coding sequences (CDSs). MNase ChIP-seq data revealed RSC-bound nucleosome fragments were very heterogenous (?80 bp to 180 bp) compared sharper profile displayed inputs (140 160 bp), supporting idea promotes accessibility nucleosomal DNA. Notably, binding +1 and CDSs, but not with -1 nucleosomes, strongly correlated Pol occupancies, suggesting enrichment CDSs linked transcription. also observed associates throughout similar RSC, II-protected highly heterogenous, consistent interacts remodeled CDSs. This supported observation genes harboring high-levels their most affected ablating function. Additionally, rapid nuclear depletion Sth1 decreases results accumulation slower clearance elongating cells reduced function, distinct from effect on PIC assembly. Altogether, our provide evidence support role promoting elongation, addition its regulating initiation.