The reaction of protein thiol groups with some disulphides

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The reaction of protein thiol groups with some disulphides.

Rapport, D. & Canzanelli, A. (1950). Science, 112, 469. Roffo, A. & Correa, L. (1938). Rev. sudamer. Endocr. 20, 548. Schmidt, G. & Thannhauser, S. J. (1945). J. biol. Chem. 161, 83. Schneider, W. C. (1945). J. biol. Chem. 161, 293. Smith, I. (1958). Chromatographic and Electrophoretic Technique8. London: William Heinemann (Medical Books) Ltd. Spiegel-Adolf, M. (1935). J. Amer. chem. Soc. 57, 1...

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The thiol groups of fumarase.

Several reagents have been employed to determine the role of the thiol groups in fumarase. The nature of the reaction of these reagents indicates that the thiol groups are not associated with active site structures but are buried in a hydrophobic environment in the interior of the enzyme. Several observations are in accord with this view. The rate of reaction of the thiol groups is much slower ...

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The Reversible Reaction of Protein Amino Groups

1. The reaction of exo-ci8-3,6-endoxo-A4-tetrahydrophthalic anhydride with amino groups ofmodel compounds and lysozyme is described. 2. Reaction with the e-amino group of Na-acetyl-L-lysine amide gives rise to two diastereoisomeric products; at acidpH the free amino group is liberated with anchimeric assistance by the neighbouring protonated carboxyl group with a half-time of 4-5h at pH 3.0 and...

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Reaction of fluorescein isothiocyanate with thiol and amino groups of sarcoplasmic ATPase.

Several model compounds containing thiol and/or amino groups (mercaptoethanol, glutathione, cysteine, ethanolamine, glycine) were studied with respect to their reactivity towards fluorescein isothiocyanate (followed spectrophotometrically at 504 and 412 nm), stability of product and long-wave absorption maximum of the fluorescein residue attached. Thiol groups reacted by far more readily than a...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1963

ISSN: 0006-2936

DOI: 10.1042/bj0880084