The Primary Structure of Staphylococcal Enterotoxin B
نویسندگان
چکیده
منابع مشابه
The primary structure of staphylococcal enterotoxin B. II. Isolation, composition, and sequence of chymotryptic peptides.
Oxidized and reduced carboxymethylated enterotoxin B was digested with chymotrypsin. The peptides were fractionated on AGl-X2 and Dowex 5OW-X2, and further purified by paper chromatography and electrophoresis. A total of 67 pure peptides was isolated and their sequences were determined completely or partially. These peptides provide important overlaps of tryptic peptides previously isolated and...
متن کاملNeutralization of Staphylococcal enterotoxin B by aptamer
12 Staphylococcal enterotoxin B (SEB) is a major virulence factor for 13 Staphylococcal toxic shock syndrome (TSS). SEB activates a large subset of the T 14 lymphocytic population, releasing pro-inflammatory cytokines. Blocking 15 SEB-initiated toxicity may be an effective strategy for treating TSS. Using a 16 systematic evolution of ligands by exponential enrichment (SELEX) process, we 17 iden...
متن کاملPurification of Type B Staphylococcal Enterotoxin.
Frea, James I. (University of Wisconsin, Madison), Elizabeth McCoy, and F. M. Strong. Purification of type B staphylococcal enterotoxin. J. Bacteriol. 86:1308-1313. 1963.-A procedure for the production and isolation of type B staphylococcal enterotoxin in yields up to 4 mg per liter of toxic culture is presented. Staphylococcus aureus S6 was grown in shake flask cultures at 37 C for 24 hr in a ...
متن کاملHydrogen ion equilibrai of staphylococcal enterotoxin B.
Potentiometric and spectrophotometric titrations of staphylococcal enterotoxin B have been carried out in 0.16 M KC1 at 25”. Potentiometric titration results indicate that the titration curves are essentially reversible between pH 1.50 and 11.0. All of the ionizable groups in enterotoxin B found from the titration curves are consistent with the amino acid composition which has been reported in ...
متن کاملGenetic Fingerprinting of Staphylococcal Enterotoxin B
Staphylococcal enterotoxin B (SEB), an exotoxin produced by staphylococcal aureus, is commonly associated with food poisoning and capable of triggering flu-like symptoms resembling those of endotoxins – such as lipopolysaccharide (LPS) released from gram-negative bacterial cell walls. By comparing the host response to these distinct toxins on a genetic level, a set of genes can be identified to...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1970
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)62955-2