The Preparation of 'Monoprecipitin' Antisera to Herpes Virus Specific Antigens

Preparation of Type-specific Antisera to Reoviruses.

Preparation of type-specific antisera to reoviruses has been handicapped by the ubiquitous distribution of these viruses and frequent occurrence of reovirus antibodies in many species of animals. Antisera prepared in rhesus monkeys at this laboratory have shown extensive crossings between the three types (Table 1). Similar crossreactions have been observed in antisera prepared in rabbits (A. M....

Preparation and characterization of specific antisera to individual glycoprotein antigens comprising the major glycoprotein region of herpes simplex virus type 1.

The major glycoprotein complex (VP123) of herpes simplex virus type 1 resolved by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis was purified and further fractionated into two major and two minor components by chromatography of the isolated VP123 region on SDS-hydroxylapatite columns. The two major components (gC and gA/gB) were purified free of other polypeptides and used to p...

Studies on the Production of Specific Hyperimmune Antisera Against Type A F.M.D. Virus

Detection of specific antigens of Newcastle disease virus using an absorbed Western blotting method

Newcastle disease virus (NDV) is an economically important poultry pathogen with a worldwide distribution that may infect a wide range of domestic and wild avian species. The identification of different pathotypes of NDVs plays an important role in the diagnosis and development of vaccines to control and eradicate NDV infections. In our previous study, we showed that mono-specific antibodies ca...

studies on the production of specific hyperimmune antisera against type a r.m.d. virus

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