The Phosphate Transporter from Pea Mitochondria (Isolation and Characterization in Proteolipid Vesicles)
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چکیده
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Isolation and characterization of the tricarboxylate transporter from pea mitochondria.
The tricarboxylate transporter was solubilized from pea (Pisum sativum) mitochondria with Triton X-114, partially purified over a hydroxylapatite column, and reconstituted in phospholipid vesicles. The proteoliposomes exchanged external [(14)C]citrate for internal citrate or malate but not for preloaded d,l-isocitrate. Similarly, although external malate, succinate, and citrate competed with [(...
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A library of monoclonal antibodies directed against the proteins of the inner mitochondrial membrane was screened for antibodies that could bind to the glutamate/aspartate transporter of pea mitochondria and thereby inhibit its activity. One antibody, 2C7, had the property of inhibiting glutamate and aspartate-dependent oxaloacetate metabolism by pea mitochondria without affecting the metabolis...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1994
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.105.1.47