منابع مشابه
Mapping of drebrin binding site on F-actin.
Drebrin is a filament-binding protein involved in organizing the dendritic pool of actin. Previous in vivo studies identified the actin-binding domain of drebrin (DrABD), which causes the same rearrangements in the cytoskeleton as the full-length protein. Site-directed mutagenesis, electron microscopic reconstruction, and chemical cross-linking combined with mass spectrometry analysis were empl...
متن کاملTransient kinetic analysis of rhodamine phalloidin binding to actin filaments.
We have characterized the binding of rhodamine phalloidin to actin filaments and actin filaments saturated with either myosin subfragment-1 or tropomyosin in 50 mM KCl, 1 mM MgCl2 buffer at pH 7.0. Direct transient kinetic measurements of rhodamine phalloidin binding to actin filaments indicate an association rate constant of 2.8 x 10(4) M-1 s-1 and a dissociation rate constant of 4.8 x 10(-4) ...
متن کاملCoronin 1C harbours a second actin-binding site that confers co-operative binding to F-actin
Dynamic rearrangement of actin filament networks is critical for cell motility, phagocytosis and endocytosis. Coronins facilitate these processes, in part, by their ability to bind F-actin (filamentous actin). We previously identified a conserved surface-exposed arginine (Arg(30)) in the β-propeller of Coronin 1B required for F-actin binding in vitro and in vivo. However, whether this finding t...
متن کاملThe effect of glutaraldehyde and phalloidin on the conformation of F-actin.
Recent data allow the assertion that conformational changes in F-actin regulate actin-myosin interaction in muscle fibre (Adelstein and Eisenberg 1980; Lednev 1980; Yanagida and Oosawa 1980; Borovikov et al. 1982; Borovikov and Gusev 1983 ; Borovikov and Karandashov 1983). However, the exact mechanism of such regulation cannot be established without further experimental evidence. The study of c...
متن کاملEffects of cytochalasin and phalloidin on actin
C YTOCHALASINS and phalloidins are two groups of small, naturally occurring organic molecules that bind to actin and alter its polymerization. They have been widely used to study the role of actin in biological processes and as models for actin-binding proteins. Functionally , cytochalasins resemble capping proteins, which block an end of actin filaments, nucleate polymerization, and shorten fi...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 1985
ISSN: 0261-4189
DOI: 10.1002/j.1460-2075.1985.tb04008.x