The Pathology and Splenic Transcriptome Profiling of Trionyx sinensis Challenged with Bacillus cereus

The pathogenic Bacillus cereus strain XS0724 isolated from China can cause high lethality to Trionyx sinensis, but little information is available on their detailed interactions. In this study, histopathologic profiling indicated that B. caused vacuolization and cell necrosis in the liver, spleen, kidney, intestine. identification of virulence factor genes non-hemolytic enterotoxin (Nhe), hemolysin BL (Hbl), FM (entFM) confirmed bacterial pathogenicity. Splenic transcriptomic sequencing at 96 h post-infection identified various immune-related mapped diverse gene families, including interleukin, complement, chemokine, interferon. differentially expressed (DEGs) were enriched 2174 GO terms: 1694 biological processes, 138 cellular components, 342 molecular functions. Further KEGG enrichment DEGs primarily associated with phagosome, NF-kappa B signaling pathway, PI3K-Akt pathway. pathways may be involved elimination invasive cereus. These data laid foundation for elucidating potential mechanisms infection process, provided robust genetic evidence subsequent work resistance T. sinensis.