The pathogenesis of bacterial keratitis: studies with Pseudomonas aeruginosa

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The pathogenesis of bacterial keratitis: studies with Pseudomonas aeruginosa.

Bacterial keratitis is a sight-threatening corneal disease that is most commonly associated with the extended wear of soft contact lenses. Over the past decade, we have investigated the pathogenesis of infectious keratitis involving the opportunistic pathogen Pseudomonas aeruginosa. Our research has focused on understanding the respective roles of bacteria and host in the establishment of this ...

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Glycyrrhizin Reduces HMGB1 and Bacterial Load in Pseudomonas aeruginosa Keratitis

Purpose High mobility group box 1 (HMGB1) contributes to poor disease outcome in Pseudomonas aeruginosa keratitis. This study tests the prophylactic effect of treatment with HMGB1 inhibitors, glycyrrhizin (GLY) and its derivative, carbenoxolone (CBX), for Pseudomonas keratitis. Methods We treated C57BL/6 (B6) mice subconjunctivally with GLY or CBX, infected with a noncytotoxic clinical isolat...

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Pseudomonas aeruginosa and a Proteomic Approach to Bacterial Pathogenesis

Pseudomonas aeruginosa is a Gram-negative bacterium that is ubiquitous in the environment and can cause a variety of diseases in compromised patients. The genome of P. aeruginosa strain PAO1 has been reported to contain 5570 potential proteins. The value of this genomic database is that new proteins can be recognized to use as diagnostic markers, novel drug targets, and to better understand the...

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Proteomic analysis of keratitis-associated Pseudomonas aeruginosa

PURPOSE To compare the proteomic profile of a clinical isolate of Pseudomonas aeruginosa (P. aeruginosa) obtained from an infected cornea of a contact lens wearer and the laboratory strain P. aeruginosa ATCC 10145. METHODS Antibiotic sensitivity, motility, biofilm formation, and virulence tests were performed using standard methods. Whole protein lysates were analyzed with liquid chromatograp...

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PURPOSE To describe 3 cases of culture-positive P. aeruginosa keratitis, who presented with a focus having serrated margins. METHODS Observational case report. RESULTS Three cases wearing contact lenses complained of symptoms such as pain, redness, and a decrease in vision. Slit-lamp biomicroscopic examination revealed a focus with serrated margins in all cases. The patients underwent corne...

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ژورنال

عنوان ژورنال: Clinical and Experimental Optometry

سال: 2002

ISSN: 0816-4622,1444-0938

DOI: 10.1111/j.1444-0938.2002.tb03082.x