The Nucleotide Sequence of a Streptomycin 6-Phosphotransferase Gene from a Streptomycin Producer

Molecular cloning and expression in Streptomyces lividans of a streptomycin 6-phosphotransferase gene from a streptomycin-producing microorganism.

The gene encoding streptomycin 6-kinase involved in the self-resistance of the streptomycin-producing Streptomyces griseus HUT 6037 was cloned in the plasmid vector pIJ703. The resulting plasmid, pSP6, contained 2.5 kb inserts of S. griseus DNA. When streptomycin-susceptible S. lividans 1326 was retransformed with pSP6, all transformants produced streptomycin 6-kinase. Addition of streptomycin ...

Streptomycin resistance in a streptomycin-producing microorganism.

Cell-free extracts of Streptomyces bikiniensis contain an adenosine 5'-triphosphate-dependent kinase which inactivates streptomycin (Sm) and dihydrostreptomycin by phosphorylation. The products have been identified as streptomycin 6-phosphate and dihydrostreptomycin 6-phosphate. Activity was not present in logarithmic-phase cells, which were susceptible to 25 mug of Sm per ml. In stationary-pha...

Streptomycin

ever, certain adjuvants, e.g. para-aminosalicylic acid, may delay the development of drug-resistance. Streptomycin is not absorbed from the alimentary tract and, therefore, it must always be given intramuscularly as well as directly, where possible, into the spaces which it is desired to render free from bacteria. The drug is excreted by the kidney. After intramuscular injection an adequate blo...

Streptomycin

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Streptomycin