THE NINHYDRIN REACTION WITH AMINO-ACIDS AND AMMONIUM SALTS

The Ninhydrin Reaction with Amino-acids and Ammonium Salts.* by Victor

Among the many biological reactions which have attracted attention during recent years, the one bearing the name of Abderhaldenl has been prominent. The simple nature of the underlying idea, that a circulating foreign protein induces in the blood a corresponding and specific defensive ferment which accomplishes its hydrolysis, combined with the obvious importance of its applications, has render...

ON THE MECHANISM OF THE REACTION OF NINHYDRIN WITH a-AMINO ACIDS

Our purpose is to show by means of a quantitative method for determination of hydrindantin derivatives that previous concepts of the mechanism of the reaction of ninhydrin with amino acids are inadequate or erroneous, and to show facts supporting a new concept. The main facts are as follows: (a) Hydrindantin forms when amino acids react with ninhydrin (Ruhemann (1) ; Abderhalden (2)) ; (b) it r...

The Ninhydrin Reaction with Amines and Amides

1 Harding, V. J., and Warneford, F. H. S., J. Biol. Chem., 1916, xxv, 319. 2 Dakin, H. D., and Dudley, H. W., J. Biol. Chem., 1913, xv, 127. 3 Neuberg, C., Biochem. Z., 1913, lvi, 500. 4 Ruhemann, S., J. Chem. Sot. 1910, xcvii, 2026. 5 In this and the preceding paper triketohydrindene hydrate has sometimes been written as the triketone for the sake of simplicity in explaining the decompositions...

Determination of Free Amino Acids by Titra- Tion of the Carbon Dioxide Formed in the Reaction with Ninhydrin

In the present method the CO2 evolved by decarboxylating amino acids with ninhydrin is transferred to standard barium hydroxide and titrated. The conditions for making the ninhydrin reaction quantitative are those previously worked out for applicat,ion in the manometric method (6, 7). The transfer of the CO, to the barium hydroxide is done by a distillation in vacua which is completed in 2 to 3...

Complex Salts of Amino Acids and Peptides