منابع مشابه
The molten globule state is unusually deformable under mechanical force.
Recently, the role of force in cellular processes has become more evident, and now with advances in force spectroscopy, the response of proteins to force can be directly studied. Such studies have found that native proteins are brittle, and thus not very deformable. Here, we examine the mechanical properties of a class of intermediates referred to as the molten globule state. Using optical trap...
متن کاملBovine serum fetuin is unfolded through a molten globule state.
The reversible heat- and GuHCl-induced unfolding of bovine serum fetuin (BSF) has been studied by differential scanning calorimetry, circular dicroism, tryptophan fluorescence, and size-exclusion chromatography. We show here that thermal unfolding of BSF occurs in two distinct steps corresponding to transitions from the native (N) to an intermediate (I) and from the intermediate to the unfolded...
متن کاملSugar-induced molten-globule model.
Proteins denature at low pH because of intramolecular electrostatic repulsions. The addition of salt partially overcomes this repulsion for some proteins, yielding a collapsed conformation called the A-state. A-states have characteristics expected for the molten globule, a notional kinetic protein folding intermediate. Here we show that the addition of neutral sugars to solutions of acid-denatu...
متن کاملConformational selection in the molten globule state of the nuclear coactivator binding domain of CBP.
Native molten globules are the most folded kind of intrinsically disordered proteins. Little is known about the mechanism by which native molten globules bind to their cognate ligands to form fully folded complexes. The nuclear coactivator binding domain (NCBD) of CREB binding protein is particularly interesting in this respect as structural studies of its complexes have shown that NCBD folds i...
متن کاملFirst Observation of the Molten Globule State of a Single Homopolymer Chain.
In a recent laser light-scattering study on the “globule-to-coil” transition (melting) of a single poly(Nisopropylacrylamide) (PNIPAM) linear chain in water (the solution is in the stable one-phase region at all temperatures), we observed that at the initial melting stage the ratio of the hydrodynamic radius to the radius of gyration underwent an unexpected maximum s,1.61d which is even higher ...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 1996
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.10.1.8566530