The methylotrophic yeast Hansenula polymorpha: a versatile cell factory
نویسندگان
چکیده
منابع مشابه
The methylotrophic yeast Hansenula polymorpha: its use in fundamental research and as a cell factory.
During the last decade methylotrophic yeasts have gained increasing interest, both for fundamental (van der Klei and Veenhuis, 1996) and applied purposes (van Dijk et al., 2000; Gellissen, 2000). The special value of these yeasts (mainly Pichia pastoris and Hansenula polymorpha) in fundamental research is undoubtedly related to studies on the principles of peroxisome homeostasis (biogenesis vs....
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BACKGROUND Currently, the two most commonly used fibrinolytic agents in thrombolytic therapy are recombinant tissue plasminogen activator (rt-PA) and streptokinase (SK). Whereas SK has the advantage of substantially lower costs when compared to other agents, it is less effective than either rt-PA or related variants, has significant allergenic potential, lacks fibrin selectivity and causes tran...
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The dramatic expansion of the peroxisomal compartment known to occur in the methanol-utilizing yeast Hansenula polymorpha on transfer from glucose- to methanol-containing media was shown to be accompanied by the synthesis of at least six major polypeptides that dominate the polypeptide pattern of total cell extracts analyzed by NaDodSO(4)/polyacrylamide gel electrophoresis. Two of these polypep...
متن کاملA novel platform for the production of nonhydroxylated gelatins based on the methylotrophic yeast Hansenula polymorpha.
The use of yeast as a host for heterologous expression of proteins that are normally derived from animal tissue is a promising way to ensure defined products that are devoid of potential harmful animal side products. Here we report on the production and secretion of a custom-designed gelatin, Hu3-His8, by the yeast Hansenula polymorpha. We observed that Hu3-His8 was poorly secreted by the heter...
متن کاملBioconversion of airborne formaldehyde by immobilized formaldehyde dehydrogenase from the recombinant methylotrophic yeast Hansenula polymorpha
Formaldehyde (FA) is a very toxic pollutant. FA in indoor air has a negative effect on human health and should be removed by ventilation or by conversion to non-toxic products. The formaldehyde dehydrogenase (FdDH), a NADand glutathione-dependent enzyme from recombinant methylotrophic yeast Hansenula polymorpha, was tested for its ability to oxidize airborne FA. A continuous fluidized bed biore...
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ژورنال
عنوان ژورنال: Enzyme and Microbial Technology
سال: 2000
ISSN: 0141-0229
DOI: 10.1016/s0141-0229(00)00173-3