The messenger ribonucleic acid content of Bacillus subtilis 168

The stability of messenger ribonucleic acid during sporulation in Bacillus subtilis.

The stability of postlogarithmic mRNA has been assessed in Bacillus subtilis during sporulation time periods. The appearance of refractile bodies, alkaline phosphatase synthesis, and total protein synthesis is controlled by mRNA which has an estimated half-life of less than 10 min. Inhibition of RNA synthesis at various times by rifampin addition or temperature shift with a temperature-sensitiv...

Lipiarmycin-resistant ribonucleic acid polymerase mutants of Bacillus subtilis.

Lipiarmycin inhibited the activity of deoxyribonucleic acid-dependent ribonucleic acid (RNA) polymerase in vitro. We showed that inhibition was due to interference by lipiarmycin with the activity of sigma-containing molecules of RNA polymerase. Transcription by core enzyme was relatively resistant to the drug, but addition of sigma led to highly drug-sensitive RNA synthesis. We isolated lipiar...

Excretion and Degradation of Ribonucleic Acid by Bacillus Subtilis.

Demain, A. L. (Merck & Co., Inc., Rahway, N.J.), R. W. Burg, and D. Hendlin. Excretion and degradation of ribonucleic acid by Bacillus subtilis. J. Bacteriol. 89:640-646. 1965.-Bacillus subtilis MB-1480 was found to produce several 5'-ribonucleotides in the extracellular medium, in addition to the previously found 5'-guanosine monophosphate and guanosine diphosphate. The nucleotides accumulated...

Suppressor system in Bacillus subtilis 168.

Multiple auxotrophic strains of Bacillus subtilis 168 were tested for joint one-step reversion of two or more auxotrophic markers to the wild-type phenotype. Mu8u5u5, a strain requiring leucine, methionine, and threonine, yielded revertants that grew without added methionine or threonine and proved to have a suppressor gene. When transferred by transformation with deoxyribonucleic acid, this su...

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