The major outer membrane protein OprG ofPseudomonas aeruginosacontributes to cytotoxicity and forms an anaerobically regulated, cation-selective channel
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منابع مشابه
The major outer membrane protein OprG of Pseudomonas aeruginosa contributes to cytotoxicity and forms an anaerobically regulated, cation-selective channel.
OprG of Pseudomonas aeruginosa is a member of the very large and widely distributed but poorly characterized OmpW (PF0392) family of outer membrane proteins. It was established here that OprG was highly transcribed in anaerobic environments rich in iron via the ANR regulator. In the absence of OprG, P. aeruginosa was significantly less cytotoxic toward human bronchial epithelial cells. Planar b...
متن کاملIdentification of oprG, a gene encoding a major outer membrane protein of Pseudomonas aeruginosa.
Sir, Pseudomonas aeruginosa expresses between five and nine major outer membrane proteins (OMPs), depending on the conditions of growth; the genes encoding all but one of these, OprG, have been identified. OprG has an apparent molecular weight of 25,000 when outer membranes are heated to 88°C for 10 min in solubilization buffer before being subjected to SDS–PAGE and 19,000 when they are not pre...
متن کاملBordetella pertussis major outer membrane porin protein forms small, anion-selective channels in lipid bilayer membranes.
The major outer membrane protein of molecular weight 40,000 (the 40K protein) of a virulent isolate of Bordetella pertussis was purified to apparent homogeneity. The purified protein formed an oligomer band (of apparent molecular weight 90,000) on sodium dodecyl sulfate-polyacrylamide gels after solubilization at low temperatures. The porin function of this protein was characterized by the blac...
متن کاملThe major anaerobically induced outer membrane protein of Neisseria gonorrhoeae, Pan 1, is a lipoprotein.
Pan 1 is an acidic outer membrane protein of Neisseria gonorrhoeae that is expressed only when gonococci are grown anaerobically. On silver-stained sodium dodecyl sulfate-polyacrylamide gels, Pan 1 migrates as an intense but diffuse 54-kDa protein. The deduced amino acid sequence of Pan 1 from the aniA (anaerobically induced protein) open reading frame reveals a lipoprotein consensus sequence, ...
متن کاملDistribution of a protein antigenically related to the major anaerobically induced gonococcal outer membrane protein among other Neisseria species.
The Pan 1 protein of Neisseria gonorrhoeae is a novel 54-kDa outer membrane protein expressed only when gonococci are grown in the absence of oxygen. It is a major antigen recognized by sera from patients with gonococcal infection. We raised mouse monospecific polyclonal antiserum to gel-purified Pan 1 from gonococcal strain F62. The antiserum was broadly cross-reactive among gonococcal strains...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2009
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.2009.01651.x