The Lipidic Cubic Phase as a Membrane Mimetic
نویسندگان
چکیده
منابع مشابه
Femtosecond crystallography of membrane proteins in the lipidic cubic phase.
Despite recent technological advances in heterologous expression, stabilization and crystallization of membrane proteins (MPs), their structural studies remain difficult and require new transformative approaches. During the past two years, crystallization in lipidic cubic phase (LCP) has started gaining a widespread acceptance, owing to the spectacular success in high-resolution structure deter...
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School of Chemistry, Bio21 Institute, Unive CSIRO Manufacturing Flagship, Bag 10, Cla [email protected] School of Science, College of Science, En Melbourne, VIC 3000, Australia † Electronic supplementary informatio parameters for top vs. bottom well (Fig from tting a rst order decay function t gA0 concentration (mol%) (Table S1); c encapsulated in monoolein and phytantr combination of the ...
متن کاملLipid cubic phase as a membrane mimetic for integral membrane protein enzymes.
The lipidic cubic mesophase has been used to crystallize important membrane proteins for high-resolution structure determination. To date, however, no integral membrane enzymes have yielded to this method, the in meso. For a crystal structure to be meaningful the target protein must be functional. Using the in meso method with a membrane enzyme requires that the protein is active in the mesopha...
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This paper presents a plug-based microfluidic system to dispense nanoliter-volume plugs of Lipidic Cubic Phase (LCP) material and subsequently merge the LCP plugs with aqueous plugs. This system was validated by crystallizing membrane proteins in lipidic mesophases, including LCP. This system allows for accurate dispensing of LCP material in nanoliter volumes, prevents inadvertent phase transit...
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Membrane proteins play vital roles in the life of the cell and are important therapeutic targets. Producing them in large quantities, pure and fully functional is a major challenge. Many promising projects end when intractable aggregates or precipitates form. Here we show how such unfolded aggregates can be solubilized and the solution mixed with lipid to spontaneously self-assemble a bicontinu...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2011
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2011.02.057