The isolation and characterization of a lethal protein from kintoki beans (Phaseolus vulgaris).
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چکیده
منابع مشابه
The isolation and characterization of a trypsin inhibitor from Kintoki bean (Phaseolus vulgaris).
A trypsin inhibitor was isolated from beans of Phaseolus vulgaris, cultivar. Kintoki, and the specific activity increased 200 times as high as that of the crude extract. It was homogeneous on several electrophoreses and the molecular weight was about 13,000. The amino acid composition was characterized by high ratios of cystine, aspartic acid, and serine. It inhibited trypsin in a molar ratio o...
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A protein fraction which inhibits growth of weanling rats was obtained from Phaseolus vulgaris seeds of the Sanilac variety by extraction with sodium chloride solution and removal of extraneous materials by precipitation at pH 4.0 and absorption on I : I bentonite-Celite. The protein was precipitated by 0.75 saturation with ammonium sulfate. The growth inhibiting protein fraction contained at l...
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The metabolic activities of plants are dramatically altered upon mechanical injury or pathogen attack. A large number of proteins accumulate at wound or infection sites, including a heterogeneous class of proteins termed pathogenesis-related proteins (Carr and Klessig, 1990). We report here the isolation of a cDNA clone designated PvPR4 encoding a nove1 protein that resembles other pathogenesis...
متن کاملPurification and Characterization of a Lectin from Phaseolus vulgaris cv. (Anasazi Beans)
A lectin has been isolated from seeds of the Phaseolus vulgaris cv. "Anasazi beans" using a procedure that involved affinity chromatography on Affi-gel blue gel, fast protein liquid chromatography (FPLC)-ion exchange chromatography on Mono S, and FPLC-gel filtration on Superdex 200. The lectin was comprised of two 30-kDa subunits with substantial N-terminal sequence similarity to other Phaseolu...
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It has been known since 1920 (Johns & Finks, 1920) that the navy bean is an inferior source of protein for rats. Everson & Heckert (1944) reported that raw navy beans were injurious to rats when given at a 10% protein level. They also reported that autoclaving the beans destroyed the injurious effect. Kakade & Evans (1963a, b) confirmed this report and observed that rats given 10% protein as ra...
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ژورنال
عنوان ژورنال: Journal of Nutritional Science and Vitaminology
سال: 1977
ISSN: 0301-4800,1881-7742
DOI: 10.3177/jnsv.23.525