The interaction of ethidium with synthetic double-stranded polynucleotides at low ionic strength
نویسندگان
چکیده
منابع مشابه
Induction of interferon synthesis by synthetic double-stranded polynucleotides.
It has been shown recently that double-stranded ribonucleic acid (RNA) from different sources stimulates the release of interferon into the circulatory system when injected intravenously into rabbits. The same RNA preparations protected mice from viral infections and prevented the viral cytopathic effect in cultures of rabbit kidney cells (A. K. Field et al., Proc. Natl. Acad. Sci. U.S. 58:2102...
متن کاملSpectroscopic Studies on the Interaction of Co(II) Tetrapyridinoporphyrazine with Synthetic Polynucleotides and DNA
Interactions of cationic tetrakis (N,N´,N´´,N´´´-tetramethyltetra-3,4-pyridinoporphyrazinatocobalt(II) (Co(tmtppa)) with synthetic polynucleotides, poly(A-T), poly(G-C) and calf thymus DNA have been characterized in 5 mM phosphate buffer, pH 7.2, by optical absorption and fluorescence spectroscopy. The appearance of hypochromicity effect and the red shift in UV-Vis spectrum of porphyrazine was ...
متن کاملDouble-Stranded Polynucleotides as Interferon Inducers
A discussion of factors considered influential in making a polynucleotide an efficient inducer of interferon was presented. These factors were double-strandedness of the polynucleotides, the sugar moiety of the polynucleotides, thermal stability, resistance to enzymatic degradation, and molecular size of the polynucloetides. Recent developments concerning interferon induction during virus infec...
متن کاملThe interaction of actin with myosin and heavy meromyosin in solution at low ionic strength.
The interaction of actin with myosin and heavy meromyosin was studied in the presence of Mgf+ ion and adenosine triphosphate at low ionic strength. When the heavy meromyosin ATPase was 17-fold activated by actin, the actoheavy meromyosin complex responsible for this activation did not give rise to a measurable increase in the viscosity of the sample above that predicted for a noninteracting mix...
متن کاملSpecific Murine B-cell Activation by Synthetic Single- and Double-stranded Polynucleotides
The synthetic single- and double-stranded polynucleotides, poly I, poly C, and poly I.C, were shown to induce thymidine incorporation in six inbred strains of murine spleen cells. This stimulation was shown to be secondary to B-cell activation and not due to contamination of the polynucleotides with bacterial lipopolysaccharide (LPS). The ability of poly I.C to act as a B-cell mitogen, in addit...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1978
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/5.1.161