The InBIO barcoding initiative database: DNA barcodes of Iberian Trichoptera, documenting biodiversity for freshwater biomonitoring in a Mediterranean hotspot

The Trichoptera are an important component of freshwater ecosystems. In the Iberian Peninsula, 380 taxa caddisflies known, with nearly 1/3 total species being endemic in region. A reference collection morphologically identified specimens, representing 142 taxa, was constructed. InBIO Barcoding Initiative (IBI) 01 dataset contains records 438 sequenced specimens. this correspond to about 37% diversity. Specimens were collected between 1975 and 2018 deposited IBI at CIBIO (Research Center Biodiversity Genetic Resources, Portugal) or Marcos A. González University Santiago de Compostela (Spain). Twenty-nine species, from nine different families, new additions Barcode Life Data System (BOLD). success identification rate over 80% achieved when comparing morphological identifications DNA barcodes for analysed. This encouraging step advances incorporation informed Environmental tools biomonitoring schemes, given shortcomings larvae adult Caddisflies such studies. barcoding not successful identifying six genera: Hydropsyche (Hydropsychidae), Athripsodes (Leptoceridae), Wormaldia (Philopotamidae), Polycentropus (Polycentropodidae) Rhyacophila (Rhyacophilidae) Sericostoma (Sericostomatidae). high levels intraspecific genetic variability found, combined a lack barcode gap challenging identification, rendered these as needing additional studies resolve their taxonomy.