The impaired development of sheep ICSI derived embryos is not related to centriole dysfunction

Abstract While intracytoplasmic sperm injection (ICSI) is an asset in human Assisted Reproduction Technologies (ART), its outcomes, terms of blastocyst, still unacceptably low ruminants. The picture typically found ICSI derived bovine and ovine embryos asymmetry between a high activation rate, marked by pronuclear development, first cleavage rate. Abnormal centriole function has been indicated as possible factor which undermines embryonic development following ICSI, especially when Freeze Dried spermatozoa (FD) are used. In order to verify the hypothesis that dysfunction might be responsible for outcomes sheep, we have investigated micro-tubular dynamics, markedly aster nucleation, fertilized sheep zygotes with frozen/thawed (FT) FD spermatozoa; In Vitro Fertilized (IVF) oocytes were used control. nucleation was assessed at different time points IVF immune-detection ?-tubulin. Pronuclear stage, syngamy embryo assessed. No difference noticed timing microtubule elongation ICSI-FT control ones, while delay recorded ICSI-FD ones. proportion 2-pronuclear stage similar (47% 53%, respectively), both much lower comparing ones (73%). Likewise, observed minority groups (28.5% vs 12.5% ICSI-FT/FD respectively) controls (50%), number blocked (71.5% 87.5% respectively). no significant differences rate ICSI-FD, (31%, 34% 44%) respectively, blastocyst compromised groups, (10% ICIS-FD 19% 33% (P