THE HYDROLYSIS OF NUCLEOPROTEINS BY CATHEPSINS FROM CALF THYMUS
نویسندگان
چکیده
منابع مشابه
The hydrolysis of nucleoproteins by cathepsins from calf thymus.
A combination of enzymatic and staining cytological techniques with ultraviolet spectroscopy has suggested the possible participation of nucleoproteins in the synthetic processes of cells (l-4). Little is known concerning the function of enzymes in the intracellular metabolism of nucleoproteins. One approach to the problem has been through the study of the action of enzymes upon nucleoproteins....
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The protein glutaredoxin, required for GSH-dependent ribonucleotide reduction, has been purified to homogeneity from calf thymus. The preparative method consisted of ammonium sulfate precipitation and three chromatography steps on DEAEkellulose, Sephadex G-50, and CM-Sepharose. Calf thymus glutaredoxin was assayed on the basis of its inherent GSH-disulfide transhydrogenase activity. Glutaredoxi...
متن کاملDeoxycytidine kinase from calf thymus.
Kinetic constants were determined for 34 nucleoside substrates of deoxycytidine kinase (EC 2.7.1.74) from calf thymus. Substrate efficiency was assessed by the ratio of V,,,,. to K,. Inhibition constants were determined for 61 nonsubstrate nucleosides or nucleoside analogues. The enzyme was relatively specific for the pentose moiety of nucleoside substrates. P-D-2’-Deoxyribonucleosides were mor...
متن کاملFour different DNA helicases from calf thymus.
Using a strand displacement assay we have followed DNA helicase activities during the simultaneous isolation of several enzymes from calf thymus such as DNA polymerases alpha, delta, and epsilon, proliferating cell nuclear antigen, and replication factor A. Thus we were able to discriminate and isolate four different DNA helicases called A, B, C, and D. DNA helicase A is identical with the enzy...
متن کاملCalf thymus polymerase.
The term polymerase has been applied to the deoxyribonucleoside triphosphate-polymerizing enzyme isolated from Escherichia coli (1). Enzymes with similar activity have been purified from regenerating rat liver (2) and calf thymus gland (3). An ample and varied supply of such polymerases is desirable for studies on the mechanism of deoxyribonucleic acid synthesis. The present work describes a pa...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1949
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)56608-4