The Herpes Simplex Virus Type 1 Helicase-primase

Herpes simplex virus type 1 helicase-primase: DNA binding and consequent protein oligomerization and primase activation.

The heterotrimeric helicase-primase complex of herpes simplex virus type I (HSV-1), consisting of UL5, UL8, and UL52, possesses 5' to 3' helicase, single-stranded DNA (ssDNA)-dependent ATPase, primase, and DNA binding activities. In this study we confirm that the UL5-UL8-UL52 complex has higher affinity for forked DNA than for ssDNA and fails to bind to fully annealed double-stranded DNA substr...

Kinetic Interplay between Herpes Simplex Virus Type 1 Helicase-Primase and Polymerase

Boulder, Colorado ii Acknowledgements Special thanks to my advisor, Dr. Rob Kuchta, for his guidance, mentorship, and support in my pursuit of honors in biochemistry and my future career. I also would like to extend thanks to the rest of the Kuchta lab, especially Dr. Andrew Olson for being a wonderful and patient mentor when I first started, and Sarah Dickerson for always being available to bo...

Role of the herpes simplex virus helicase-primase complex during adeno-associated virus DNA replication.

A subset of DNA replication proteins of herpes simplex virus (HSV) comprising the single-strand DNA-binding protein, ICP8 (UL29), and the helicase-primase complex (UL5, UL8, and UL52 proteins) has previously been shown to be sufficient for the replication of adeno-associated virus (AAV). We recently demonstrated complex formation between ICP8, AAV Rep78, and the single-stranded DNA AAV genome, ...

Adeno-Associated Virus DNA Replication Helicase-Primase Complex during Role of the Herpes Simplex Virus

Herpes Simplex Virus Type 1 Single Strand DNA Binding Protein and Helicase/Primase Complex Disable Cellular ATR Signaling

Herpes Simplex Virus type 1 (HSV-1) has evolved to disable the cellular DNA damage response kinase, ATR. We have previously shown that HSV-1-infected cells are unable to phosphorylate the ATR substrate Chk1, even under conditions in which replication forks are stalled. Here we report that the HSV-1 single stranded DNA binding protein (ICP8), and the helicase/primase complex (UL8/UL5/UL52) form ...