THE GOLGI APPARATUS OF AMOEBA PROTEUS PALLAS

Cytochemical Staining of the Golgi Apparatus in Amoeba Proteus

The Golgi apparatus has been shown to be involved in the packaging of secretory proteins (Caro and Palade, 1964 ; Jamieson and Palace 1967 a, 1967 b), and in the production of acid mucopolysaccharides and glycoproteins (Neutra and Leblond, 1966 ; Berlin, 1967 ; Thiéry, 1967 ; Rambourg et al, 1969; Whur et al., 1969) . Other suggested functions of the Golgi apparatus include the formation of the...

Filaments of Amoeba Proteus

Amoeba proteus has two types of cytoplasmic filaments that are thought to be important in cell motility, cytoplasmic streaming, and changes in cytoplasmic consistency : thin, 50-70 A wide filaments, and short, thick, 160 A wide rods (Pollard and Ito, 1970) . The thin filaments resemble the actin filaments of muscle (Hanson and Lowy, 1963), Acanthamoeba (Weihing and Korn, 1969 ; Pollard et al .,...

Cytoplasmic Filaments of Amoeba Proteus

The role of filaments in consistency changes and movement in a motile cytoplasmic extract of Amoeba proteus was investigated by correlating light and electron microscopic observations with viscosity measurements. The extract is prepared by the method of Thompson and Wolpert (1963). At 0 degrees C, this extract is nonmotile and similar in structure to ameba cytoplasm, consisting of groundplasm, ...

Membrane potentials in Amoeba proteus.

Calcium distribution in Amoeba proteus

A preliminary investigation of the distribution of cellular calcium in Amoeba proteus was undertaken. Total cellular calcium under control conditions was found to be 4.59 mmol/kg of cells. When the external Ca++ concentration is increased from the control level of 0.03 to 20 mM, a net Ca++ influx results with a new steady-state cellular calcium level being achieved in integral of 3 h. At steady...