The Glucoamylase Encoding Gene from Aspergillus oryzae
نویسندگان
چکیده
منابع مشابه
Cloning and nucleotide sequence of the acid protease-encoding gene (pepA) from Aspergillus oryzae.
We have cloned a genomic DNA sequence encoding the acid protease (PEPA) from Aspergillus oryzae using a 0.6-kb fragment as a probe. This fragment was amplified by the polymerase chain reaction (PCR) using oligonucleotide primers designed from the partial amino acid sequences of peptide fragments of the purified protein. Nucleotide sequencing analysis has shown that the cloned gene (designated p...
متن کاملDeletion analysis of the catalase-encoding gene (catB) promoter from Aspergillus oryzae.
The catalase-encoding gene (catB) is expressed strongly in Aspergillus oryzae. To identify the transcription regulatory elements involved in strong expression, we did promoter deletion analysis using beta-glucuronidase (GUS) as a reporter and an electrophoretic gel mobility shift assay (EMSA) systematically. The deletion 200-bp sequence from -1,000 to -800 in the 1,400-bp catB promoter caused a...
متن کاملCloning, nucleotide sequencing, and expression of the beta-galactosidase-encoding gene (lacA) from Aspergillus oryzae.
lacA coding for beta-galactosidase (beta-gal) was cloned from the genomic DNA of Aspergillus oryzae RIB40. There were 9 exons in lacA and the coding region of 3,015 bp encoded a protein of 1,005 aa with a deduced molecular mass of 109,898. A. oryzae lacA was highly homologous to fungal beta-gals, with the highest aa identity of 70.7% to A. niger lacA, and also showed significant identity to aci...
متن کاملThe promoter of the glucoamylase-encoding gene of Aspergillus niger functions in Ustilago maydis.
Promoter sequences from the Aspergillus niger glucoamylase-encoding gene (glaA) were linked to the bacterial hygromycin (Hy) phosphotransferase-encoding gene (hph) and this chimeric marker was used to select Hy-resistant (HyR) Ustilago maydis transformants. This is an example of an Ascomycete promoter functioning in a Basidiomycete. HyR transformants varied with respect to copy number of integr...
متن کاملProduction and optimization of amylase and glucoamylase using Aspergillus oryzae under solid state fermentation
Filamentous fungi have been widely used to produce hydrolytic enzymes for industrial applications. Fungal enzymes amylase and glucoamylase were produced using a specific culture Aspergilus oryzae under solid state fermentation and the enzymes obtained were partially purified using isopropanol. Screening of four agricultural residues viz. rice bran, wheat bran, rice bran: wheat bran (1:1), rice ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: JOURNAL OF THE BREWING SOCIETY OF JAPAN
سال: 1991
ISSN: 0914-7314,2186-4012
DOI: 10.6013/jbrewsocjapan1988.86.829