The globalization of messenger RNA regulation

The globalization of messenger RNA regulation.

Transcriptional regulation has been a dominant area of research in gene expression for more than 50 years. However, the past 20 years of research in biology brought new focus on posttranscriptional events, namely RNA regulation. This emphasis on understanding the regulatory functions of RNAs emerged with the identification of cisand trans-regulatory regions in pre-mRNAs and mature mRNAs, noncod...

N6-methyladenosine-dependent regulation of messenger RNA stability

N-methyladenosine (mA) is themost prevalent internal (non-cap) modificationpresent in themessengerRNAofall higher eukaryotes. Although essential to cell viability and development, the exact role ofmAmodification remains tobedetermined.The recent discovery of twomAdemethylases inmammalian cells highlighted the importance of mA in basic biological functions and disease. Here we show that mA is se...

Location and regulation of rat angiotensinogen messenger RNA.

The presence of angiotensinogen messenger RNA (mRNA) was detected in rat vascular and adipose tissue. Angiotensinogen mRNA in rat aorta was localized in the adventitia and surrounding adipose tissue, and not in the vascular smooth muscle. Freshly dispersed and cultured endothelial and aortic smooth muscle cells did not contain detectable amounts of angiotensinogen mRNA. In addition to periaorti...

Messenger RNA.

Human ADAM33 messenger RNA expression profile and post-transcriptional regulation.

We examined transcript expression and post-transcriptional regulation of human ADAM33, a recently identified asthma gene. A detailed messenger RNA (mRNA) expression profile was obtained using Northern, reverse transcription polymerase chain reaction, and in situ hybridization analyses. ADAM33 mRNA was expressed significantly in smooth muscle-containing organs, minimally in immune organs and hem...