The FtsZ-Like Protein FtsZm of Magnetospirillum gryphiswaldense Likely Interacts with Its Generic Homolog and Is Required for Biomineralization under Nitrate Deprivation
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منابع مشابه
The Disruption of an OxyR-Like Protein Impairs Intracellular Magnetite Biomineralization in Magnetospirillum gryphiswaldense MSR-1
Magnetotactic bacteria synthesize intracellular membrane-enveloped magnetite bodies known as magnetosomes which have been applied in biotechnology and medicine. A series of proteins involved in ferric ion transport and redox required for magnetite formation have been identified but the knowledge of magnetosome biomineralization remains very limited. Here, we identify a novel OxyR homolog (named...
متن کاملFunctional Analysis of the Magnetosome Island in Magnetospirillum gryphiswaldense: The mamAB Operon Is Sufficient for Magnetite Biomineralization
Bacterial magnetosomes are membrane-enveloped, nanometer-sized crystals of magnetite, which serve for magnetotactic navigation. All genes implicated in the synthesis of these organelles are located in a conserved genomic magnetosome island (MAI). We performed a comprehensive bioinformatic, proteomic and genetic analysis of the MAI in Magnetospirillum gryphiswaldense. By the construction of larg...
متن کاملMagnetite biomineralization in Magnetospirillum gryphiswaldense: a time resolved X-ray Absorption Spectroscopy study
Magnetotactic bacteria biosynthesize magnetite nanocrystals (magnetosomes) with high chemical purity, species-specific crystal morphology on shape and size, and narrow size distribution. These magnetosomes allow the bacteria to orient and navigate along the geomagnetic field lines. The interest in these bacteria has recently increased because of the potential biomedical applications of the magn...
متن کاملIdentification of promoters for efficient gene expression in Magnetospirillum gryphiswaldense.
To develop an expression system for the magnetotactic bacterium Magnetospirillum gryphiswaldense, we compared gene expression from the widely used Escherichia coli P(lac) promoter with that from known and predicted genuine M. gryphiswaldense promoters. With the use of green fluorescent protein as a reporter, the highest expression level was observed with the magnetosomal P(mamDC) promoter. We d...
متن کاملTranscriptional organization and regulation of magnetosome operons in Magnetospirillum gryphiswaldense.
Genes involved in magnetite biomineralization are clustered within the genomic magnetosome island of Magnetospirillum gryphiswaldense. Their transcriptional organization and regulation were studied by several approaches. Cotranscription of genes within the mamAB, mamDC, and mms clusters was demonstrated by reverse transcription-PCR (RT-PCR) of intergenic regions, indicating the presence of long...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2014
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.00804-13