The effect of simvastatin treatment on TNF-alpha-induced inflammation in human vascular endothelial cells (HUVECs)

This study investigates the effects of simvastatin (SMV) on human umbilical vein endothelial cells (HUVECs) inflamed by tumor necrosis factor-alpha (TNF-a). Cells were treated with (5 μM, 10 and 50 μM) with/without TNF (20 ng/mL). The proliferation examined, its cytotoxic effect was determined lactate dehydrogenase (LDH) assays. Metalloproteinase expression (MMP-2 MMP-9) levels SMV at different concentrations qPCR, protein Western blot. increasing down-regulated MMPs. After stimulation TNF-a, wound healing time in case formation examined. results interestingly indicated that delayed closure 2.5-fold when it administered alone to HUVECs but did not change conditions. suggests inflammation is a required condition healing, absence inflammation, treatment delays vitro.