The Detection and Variation of Strawberry mottle virus
نویسندگان
چکیده
منابع مشابه
Detection of Strawberry mottle virus by molecular hybridization
Strawberry mottle virus (SMoV) is one of the viral pathogens affecting strawberries (Fragaria spp.) production severely worldwide. Currently, reverse transcription-polymerase chain reaction (RT-PCR) is frequently used to detect SMoV, which is sometimes not successful because of unsatisfied nucleic acid quality and the contamination of secondary metabolites resulting in poor PCR amplification. I...
متن کاملشناسایی مولکولی ویروس پیسک توتفرنگی (Strawberry mottle virus) از مزارع توتفرنگی استان کردستان بر اساس منطقه 3΄NCR
در تابستان 1397، علائم ویروسی شامل پیسک، بدشکلی و سوختگی حاشیه برگها از مزارع توتفرنگی استان کردستان مشاهده و تعداد 48 نمونه برگی جمعآوری شد. ابتدا برخی نمونهها به گیاه محک Fragaria vesca با استفاده از شته توتفرنگی (Chaetosiphon fragaefolii) تلقیح و علایم مطابق با Strawberry mottle virus (SMoV) ظاهر شد. سپس، آر ان ا کل از نمونهها با روش سیلیکا استخراج و دی ان ا...
متن کاملIdentification of Cleavage Sites Recognized by the 3C-Like Cysteine Protease within the Two Polyproteins of Strawberry Mottle Virus
Strawberry mottle virus (SMoV, family Secoviridae, order Picornavirales) is one of several viruses found in association with strawberry decline disease in Eastern Canada. The SMoV genome consists of two positive-sense single-stranded RNAs, each encoding one large polyprotein. The RNA1 polyprotein (P1) includes the domains for a putative helicase, a VPg, a 3C-like cysteine protease and an RNA-de...
متن کاملCorrigendum: Identification of Cleavage Sites Recognized by the 3C-Like Cysteine Protease within the Two Polyproteins of Strawberry Mottle Virus
[This corrects the article on p. 745 in vol. 8, PMID: 28496438.].
متن کاملDevelopment of SYBR Green I Based Real-Time RT-PCR Assay for Specific Detection of Watermelon silver mottle Virus
Background: Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. Objectives: Development of a highly sensitive and reliable detection method for WSMoV. Materials and Methods: Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...
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ژورنال
عنوان ژورنال: Plant Disease
سال: 2003
ISSN: 0191-2917,1943-7692
DOI: 10.1094/pdis.2003.87.4.385